There are many kinds of elisa kits in China: for example, ELISA test kit, ELISA kit, enzyme-linked immunosorbent assay kit, enzyme-linked immunosorbent assay kit, enzyme-linked immunosorbent assay kit, enzyme-free kit, etc. Commonly known as ELISA test kits, enzyme-linked immunosorbent assay kits, etc. Since the advent of the 60-70s, ELISA kits have been recognized and respected by researchers all over the world, and have been widely promoted in Europe, America and China. Especially the development of domestic biochemical field. Rat ELISA kits play an important role in the most extensive and sensitive technical means of scientific research and production. Although the operation steps seem simple, there are many operational points in the experimental process that need to be mastered. If there are slight inadvertent and unreasonable operation steps, many problems will be affected and the quality of the test will be greatly reduced.
(1) Selection of solid phase carrier: Many substances can be used as a solid phase carrier such as polyvinyl chloride, polystyrene, polyacrylamide, and cellulose. The form may be a flat plate, a test tube, a bead or the like. Currently used is a 40-well polystyrene recessed plate. Rat ELISA kits can be screened before use regardless of the carrier: coating with the same amount of antigen, reacting under the same experimental conditions, observing whether the color reaction is uniform, and judging whether the adsorption performance is good. .
(2) Selection of coated antibody (or antigen): When the antibody (or antigen) is adsorbed on the surface of the solid phase carrier, the purity is required to be good, and the pH is generally required to be between 9.0 and 9.6. The adsorption temperature, time and the amount of protein also have a certain influence, and generally use 4 ° C for 18 to 24 hours. The optimal concentration of protein coating is titrated: the rat ELISA kit is coated with different protein concentrations (0.1, 1.0, and 10 μg/ml, etc.), and the other rat ELISA kits are observed for the same conditions. The OD value of the specimen. The concentration with the highest OD value and the least amount of protein is selected. It is usually 1 to 10 μg/ml for most proteins.
(3) Selection of working concentration of enzyme-labeled antibody: First, titration of preliminary titer was carried out by direct rat ELISA kit method (see enzyme-labeled antibody fraction). Then, other conditions are fixed or the "square matrix method" (the coating, the reference sample of the sample to be tested, and the enzyme-labeled antibody are respectively different dilutions) are accurately titrated in the formal experimental system.
Shanghai Jinma Bio has been the brand Elisa kit for many years, and researched a variety of Elisa kits. We provide first-class kits for customers, all-round pre-sale, sale, after-sales service, what are you hesitating? Strict quality control system: All kits undergo strict quality control from raw materials to finished products to ensure the quality and stability of the kit. Professional testing services: WB test, ELISA kit test, IHC test, dye test (Shanghai customers can take samples).
Order the rat Elisa kit, just in Shanghai Jinma Biotech!
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