When many friends are doing Elisa experiments, they only know that improper storage of specimens will affect the experimental results. Improper washing and improper washing steps will lead to abnormal results, but today Xiaobian is not known, and the microplate reader setting parameters will also affect. the result.
For substrate determination, if the reading time is equal to or exceeds 2 sec/well, there is a time delay in reading from the first hole to the last hole, which may cause a change in the result. In the substrate reaction phase, horseradish peroxidase will continue to act until the substrate reacts completely. Due to the delay of time, the substrate is getting less and less, and the substrate reaction is also affected. The reading time of the microplate reader was set to 1.0 sec/well. Any successful ELISA test, the correct method of operation and its steps are a critical and important aspect. Great care should be taken during the experiment. Shanghai Jinma Bio has been conducting elisa kit for many years and has rich clinical experience. Our company provides comprehensive technical guidance to protect your experiment.
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